Anti-mutagenicity Effects of Vitamin E on
Oncology and Non-oncology Hospital
Nurses by Ames Assay
Published: December 1, 2013 | DOI: https://doi.org/10.7860/JCDR/2013/.3790
Majid Rezaei-Basiri, Hassan Rezazadeh, Iraj Aswadi-Kermani Mahmud Ghazi-Khansari
1. Research Scholar, Department of Pharmacology and Toxicology, School of Pharmacy, Tabriz University of Medical Sciences, Tabriz-Iran.
2. Associate Professor, Department of Pharmacology and Toxicology, School of Pharmacy, Tabriz University of Medical Sciences, Tabriz-Iran.
3. Physician, Full Professor, Haematology & Oncology Research Center, Tabriz University of Medical Sciences, Tabriz-Iran.
4. Full Professor, Department of Pharmacology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran-Iran.
Correspondence
Dr. Hassan Rezazadeh,
Associate Professor, Department of Pharmacology and Toxicology, School of Pharmacy,
Tabriz University of Medical Sciences/ Iran.
Phone: +98-411-3341315, Fax: +98-411-3344798, E-mail: rezafar81@hotmail.com
Introduction:The aim of this study is to determine the anti- mutagenic effects of Vitamin E among nurses of oncology and non-oncology hospitals exposed to chemotherapy drugs. Several studies have demonstrated that nurses occupationally exposes to cytostatic drugs.
Material and Methods:A total of 138 female nurses from oncology and non-oncology hospitals participated in the study. All urine samples of nurses before and after Vitamin E consumption (200 mg/day) were evaluated by Ames Salmonella typhimorium mutagenicity test using histidine negative of tester strain TA100 with and without S-9mix. In all steps the collected urine samples extracts were prepared using amberlit XAD-2 resins and examined for mutagenicity activity. The data of Ames assay were analyzed with Anova one way and t-test statistical.
Results:In the present study 25% of oncology nursing staff excrete carcinogenic compounds in their urine and oral consumption of Vitamin E for two weeks showed significant anti-mutagenic effects.
Discussion:It was appeared that the urinary mutagenic activity will decrease by receiving Vitamin E. However, after Vitamin E consumption there was significantly depletion of urinary mutagenic activity in urine extracts among the exposed nursing personnel.
Conclusion:We conclude that mild effects of Vitamin E against poor safety and significant adverse events among nurses handling cytotoxic drugs. There is, therefore, a need to improve the safety of the work environment, make available protective equipment, develop standard practice guidelines for oncology nurses and higher therapeutic doses of Vitamin E may be a promising compound for reducing mutagenic effects of anti- neoplastic drugs among oncology hospital nurses.
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